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. 2010 Aug 23;285(44):33662–33670. doi: 10.1074/jbc.M110.156075

FIGURE 5.

FIGURE 5.

A3R agonists accelerate the beat of wild-type and Adora3i1−/− sperm. A, Adora3+/+ and Adora3−/− testis were probed for the presence of full-length transcripts of Adora3i1 and Adora3i2 (A3i1 and A3i2). B, individual sperm were perfused for 60 s with Medium HS, then (at t = 0) for 120 s with HS containing 25 μm Cl-dAdo or 30 μm Cl-IB-MECA, then for 60 s with Medium HS fortified with 15 mm NaHCO3. Shown are mean beat frequencies for sperm of wild-type (n = 18–24 cells in three experiments) and Adora3i1−/− mice (n = 12–30 cells in three experiments), sampled at t = −60, 0, 30, 60, 90, 120, and 180 s. Mean acceleration determined by regression analysis of the initial 60 s of response to Cl-dAdo and Cl-IB-MECA. Wild-type and Adora3i1−/− sperm showed no significant difference in beat frequency after 90 s of perfusion with Cl-dAdo (p = 0.23), but the beat was slightly slower (p < 0.01) for Adora3i1−/− than for wild-type sperm after 90 s of perfusion with Cl-IB-MECA.