TABLE 2.
Expression profiles of genes known to be involved in T- and B-cell activation after H. pylori infection in WT and Ctsx−/− epithelial cells
For the complete gene list, see the Oligo GEArray® Mouse T- and B-cell activation microarray (SABiosciences Corp.). Total RNA from primary gastric epithelial cells (uninfected and infected with H. pylori) were treated under the same conditions. All images were quantified using the GeneGnome and GeneTools image scanning and analysis software (Syngene BioImaging Systems). Common gene names are indicated. Expression ratios (before/after H. pylori infection) were calculated per blot or quantitative RT-PCR, and final results are expressed as -fold changes given as a relative gene expression (according to the formula: ratio treatment/ratio control).
| Microarray |
Quantitative RT-PCR (± S.D.) |
|||
|---|---|---|---|---|
| WT | Ctsx−/− | WT | Ctsx−/− | |
| -fold | -fold | |||
| B-cell proliferation | ||||
| CD81 | 1.40 | 2.12 | 1.43 ± 0.4 | 2.01 ± 0.6 |
| Cdkn1a | 1.06 | 1.76 | 1.07 ± 0.4 | 1.60 ± 1.2 |
| Prkcd | 1.52 | 1.90 | 1.05 ± 1.0 | 1.20 ± 0.8 |
| Shb | – | 1.25 | – | – |
| B-cell differentiation | ||||
| Hmgb3 | 1.42 | 2.19 | – | – |
| Inpp5d | 1.61 | 1.70 | – | – |
| T-cell proliferation | ||||
| Ccnd3 | – | 1.90 | – | – |
| Glmn | 1.23 | 1.97 | 2.01 ± 1.2 | 1.70 ± 0.1 |
| IL-18 | 1.50 | 1.75 | 1.32 ± 0.1 | 1.59 ± 0.1 |
| IL-10 | 1.55 | 1.65 | – | – |
| Spp1 | 1.02 | 2.62 | 1.05 ± 0.9 | 3.03 ± 0.1 |
| T-cell differentiation | ||||
| Ap3b1 | – | 1.85 | 1.09 ± 0.1 | 1.52 ± 0.2 |
| Gadd45g | – | 1.55 | 0.51 ± 0.2 | 1.38 ± 0.1 |