FIGURE 4.

Anion exchange chromatography of a cell extract from S. solfataricus. A cell extract was prepared from S. solfataricus grown on d-xylose and subjected to anion exchange chromatography on a HiTrap Q-Sepharose column using 50 mm Tris-HCl, pH 8, and an elution gradient of 0–0.7 M NaCl. The elution profile (A_{280 nm}) between 0.13 and 0.35 m NaCl is shown. All fractions were assayed for gluconate dehydratase (gray columns) and xylonate dehydratase (black columns), and the total activity (μmol product per min) in each fraction is displayed. mAU, milliabsorbance units.