FIGURE 1.

F-actin formation in PHD2 knockdown cells. A, 2.1.1-16 and HeLa T-Rex cells were treated with or without 10 μg/ml Tet for 96 h. Subsequently, cells were lysed, and protein extracts were analyzed by immunoblot. B, WT HeLa cells and stably transfected control shRNA (shC) or PHD2 shRNA 1B6 and 3B7 cells were lysed, and protein extracts were analyzed by immunoblot. C, 2.1.1-16 and HeLa T-Rex cells were grown on coverslips and treated with 10 μg/ml Tet for 96 h. Subsequently, cells were fixed, and F-actin organization was analyzed by phalloidin staining. D, WT HeLa, control shRNA, 1B6, and 3B7 cells were grown on coverslips. Subsequently, cells were fixed, and F-actin organization was analyzed by phalloidin staining.