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Restoration of myotube formation by Mef2D(+)β expression in C2C12-MBNL3 cells. C2C12-MBNL3 cells (A–C), clonal pools of C2C12-MBNL3 cells expressing the indicated Mef2D isoform (D–I), and control C2C12 cells (J–L) were maintained in differentiation media for 0 or 5 days. The cells were fixed and analyzed by immunocytochemistry using an anti-myosin heavy chain mAb MF20 (green). The nuclei were visualized by DAPI staining (blue). Scale bars, 50 μm.
