TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| Strains | ||
| A1 | CP1415 but Rifr through spontaneous conversion | This work |
| CP1200 | Rx but malM511 str-1 | 24 |
| CP1415 | CP1200 but comA negative by insertion of the ermAM cassette into the ClaI site of comA | 17 |
| CP1500 | hex nov-r1 bry-r str-1 ery-r1 ery-r2 | 3 |
| EH1 | EK4166 but comE negative by replacement with a Kanr cassette via double-crossover recombinations | This work |
| EK100 | CP1415 but egb negative by transformation with genomic DNA form R262 | 25 |
| EK4166 | EK100 but hirL::pEVP3 by transformation with plasmid DNA | 25 |
| EK4167 | EK4166 but Novr by transformation with CP1500 genomic DNA | 25 |
| H1 | CP1415 but Novr by transformation with CP1500 genomic DNA | 25 |
| H2 | H1 but comE::pXF518 by transformation with R319 genomic DNA | 25 |
| H3 | H1 but lytA::pEVP3 by transformation with plasmid DNA | 25 |
| H4 | EK4166 but lytA::pFW13 by transformation with plasmid DNA | This work |
| H5 | EK100 but lytC::pEVP3 by transformation with plasmid DNA | This work |
| H6 | A1 but LytA− by transformation with genomic DNA from H3 | This work |
| R262 | R800 but negative for endogenous β-galactosidase activity (ebg negative) | 1 |
| R319 | R800 but comE::pXF518 by transformation with plasmid DNA | 1 |
| S1 | CP1415 but orf62-orf51::pEVP3 by transformation with plasmid DNA | This work |
| S. pneumoniae Rx | R36A derivative | 21 |
| Plasmids | ||
| pEVP3 | Nonreplicative vector (CmrlacZ) | 4 |
| pFW13 | Nonreplicative vector (Kmr) | 20 |