TABLE 2.
Deficient strain or DNA fragment | Pheromone added | No. of CFU of transformants/ml at OD550 of:
|
||
---|---|---|---|---|
0.1 | 0.2 | 0.4 | ||
H2a | Yes | 1.8 × 104 | 4.8 × 104 | 9.7 × 104 |
No | 2.0 × 101 | 0 | 1.0 × 101 | |
Novr DNA from CP1500b | Yes | 5.9 × 105 | 3.4 × 105 | 2.4 × 105 |
No | 0 | 0 | 1.0 × 101 |
Competence-deficient mutant lacking a functional comE gene.
One microgram of purified DNA per milliliter from novobiocin-resistant strain CP1500 was used to transform strain A1.