FIGURE 3.

Effect of VCX1 disruption on vacuolar transport of potassium and calcium. At the indicated time (white arrow), 12.5 μm CaCl₂ was added to visualize the recovery of fluorescence in the presence or absence of Vcx1p (A). At the indicated time (white arrow) 50 mm Na₂SO₄ and K₂SO₄ (B) or 100 mm of NaCl and KCl (C) was added to collapse the pH gradient across the vacuolar membrane of W303 nhx1Δvnx1Δvcx1Δ resulting in a cation-dependent H⁺ movement and alkalinization of the vesicular lumen. Traces are representative of at least three independent experiments. Baf, bafilomycin.