FIGURE 2.

Effects of ribose on spectrin glycation in erythrocytes and on membrane deformability. The membranes from erythrocytes incubated with the indicated concentrations of ribose at 37 °C for 48 h were prepared by hypotonic lysis and resealed in PBS. A, membrane proteins separated on 5% SDS-acrylamide gel and stained with CBB (upper panel) and blotted onto PVDF and probed with anti-pentosidine antibody (lower panel) are shown. B, membrane deformability of the ghosts was analyzed by ektacytometry.