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. 2010 Aug 20;285(44):33930–33939. doi: 10.1074/jbc.M110.150151

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — Validation of gene expression in CaM KD by quantitative real time PCR and immunoblotting. Cultured cortical neurons from newborn wild-type mice were infected at DIV 5 with control lentivirus or with CaM KD lentivirus expressing CaM shRNAs without or with wild-type CaM rescue mRNA and analyzed at DIV 14. A, quantitative real time PCR measurements of the mRNA levels of Syt2, Lrrtm3, Syt9, and CaM3. Note that the CaM3 mRNA levels are not rescued by expression of the rescue CaM2 cDNA. B, immunoblotting analysis of Syt1, Syt2, CaM, and VCP (used as load control).