Figure 5.

Specificity test for the library plasmids extracted from candidate clones #1 and #2. DNA plasmids isolated from candidate clones #1 and #2 were subjected to restriction digest and the library plasmid was identified. The plasmid was used to cotransfect Cdc25-2 cells with the Met expression vector encoding the original bait [i.e. ChpAc. deleted in its N-terminal domain (ChpAc.ΔN)], the Met expression vector (Met) and the Met expression vector encoding for full-length myristoylated Chp activated (Met–M–ChpAc.). Transformants were selected and used to replica plate onto appropriate plates containing galactose and lacking methionine incubated at 36°C.