Skip to main content
. 2010 Aug 23;285(44):34072–34085. doi: 10.1074/jbc.M110.137380

FIGURE 3.

FIGURE 3.

Effect of PKC isoform-specific knockdown on the expression of total cellular NKCC1 in T84 cells. T84 cell monolayers grown on Transwell supports (24-mm insert diameter) were uninfected or infected with non-targeting shLacZ-adenovirus or shPKCα-, shPKCδ-, and shPKCϵ-adenoviruses (m.o.i. = 30) for 72 h. The cell lysates were probed for proteins of PKC isoforms, NKCC1, and GAPDH by Western blotting. A, effect of PKCα knockdown on total NKCC1 protein expression in T84 cells. The top blot shows the expression levels of PKCα protein, the middle blot shows the expression levels of total NKCC1 protein, and the lower blot indicates the expression of GAPDH serving as sample loading control. B, effect of PKCδ knockdown on total NKCC1 protein expression in T84 cells. The top blot shows the expression levels of PKCδ protein. C, effect of PKCϵ knockdown on total NKCC1 protein expression in T84 cells. The top blot shows the expression levels of PKCϵ protein. The blots shown are representative of three separate experiments. Quantitation of three blots from triplicate experiments was conducted by densitometry and data were analyzed using one-way ANOVA with Tukey's multiple comparison tests after normalized to the respective GAPDH blots (D–F). Data are presented as the percentage of uninfected cells (mean ± S.E., n = 3). NS, not significant.