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. 2010 Aug 19;285(44):34106–34114. doi: 10.1074/jbc.M110.136739

FIGURE 1.

FIGURE 1.

Effect of FK866 on NAD and ATP contents in various cell cultures under basal and PARP-1-activating conditions. Concentration-dependent effects of FK866 on NAD (A) and ATP (B) contents in various cultured cell types. Basal contents of NAD and ATP contents were comparable among different cell types and comprised between 10.4 ± 4.4 and 23.5 ± 7.8 nmol/mg of protein, respectively. Time course of the effects of FK866 at 100 μm and 100 nm on NAD (C) and ATP (D) contents in HeLa cells. Effects of FK866 (100 μm, 30 min preincubation) on NAD (E) and ATP (F) contents of cultured cells exposed 1 h to the PARP-1 activating compound MNNG (100 μm). Each point/column represents the mean ± S.E. of at least three experiments conducted in duplicate.