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. 2010 Aug 19;285(44):34106–34114. doi: 10.1074/jbc.M110.136739

FIGURE 2.

FIGURE 2.

Effects of various compounds on FK866-induced NAD depletion in HeLa cells. A, effects of the PARP-1 inhibitor PHE, the MART inhibitors novobiocin (NOV) and mIBG, and the Sirt-1 inhibitor EX-257 (EX) on NAD depletion prompted by FK866 (100 μm/1 h). Each drug was preincubated 30 min at the indicated concentrations. B, additive effects of the PARP-1 inhibitor PHE (30 μm) and MART inhibitors novobiocin (NOV, 1 mm) and mIBG (100 μm) on reduction of NAD depletion prompted by FK866 (100 μm/1 h). C, effects of FK866, the PARP-1 inhibitor PHE and MART inhibitors novobiocin (NOV) and mIBG (all at 100 μm) on in vitro activity of purified PARP-1. D, effects of kynurenine (KYN, 200 μm) and the kynurenine monooxygenase inhibitor Ro-618048 (RO, 30 μm) on NAD contents in cells under control conditions or exposed to FK866 (100 μm/1 h). KYN and RO have been preincubated 60 min. Each column represents the mean ± S.E. of three experiments conducted in duplicate. A, *, p < 0.05; **, p < 0.01 versus FK866. B, *, p < 0.05; **, p < 0.01 versus control. D, *, p < 0.05; **, p < 0.01 versus control; §, p < 0.05, versus FK866. Analysis of variance and Tukey's post hoc test were used.