FIG. 1.

The 9.2-kb gene region of Gordonia sp. strain TY-5. (A) Genetic organization of the gene cluster and restriction map. The orientation of the identified genes is indicated by arrows. A kan gene (1,033 bp) was inserted into the SphI site within the prmB gene and into the BclI site within the adh1 gene. The overlapping inserts of the two plasmids, pPRM1 and pPRM2, and the inverse-PCR product representing the gene region analyzed are indicated by lines. (B) Genomic Southern analysis of PstI-digested total DNAs (5.0 μg of each) from the wild-type strain and the mutant strain with prmB disrupted, with the ³²P-labeled prmB fragment as a probe. (C) Genomic Southern analysis of PstI-digested total DNAs (5.0 μg of each) from the wild-type strain and the mutant strain with prmB disrupted, with the ³²P-labeled adh1 fragment as a probe.