FIG. 2.

(A) Northern blot analysis of the prmABCD gene cluster. A 20-μg portion of total RNA was loaded into each lane, and prmABCD transcription was detected by hybridization with ³²P-labeled prmA, prmB, prmC, and prmD fragments as probes. Total RNA was prepared from Gordonia sp. strain TY-5 cells grown on propane. (B) prmABCD transcription was detected by hybridization with a ³²P-labeled prmA fragment as the probe. Total RNAs were prepared from cells induced with methane (met), ethane (et), propane (pro), and butane (but) as described in Materials and Methods and from cells grown on succinate (suc). (C) adh1 transcription was detected by hybridization with a ³²P-labeled adh1 fragment as the probe. Total RNAs were prepared from cells grown on propane, 1-propanol (1-p), and 2-propanol (2-p). (D) adh1 transcription was detected by hybridization with a ³²P-labeled adh1 fragment as the probe. Total RNA was prepared from cells with prmB disrupted that were induced with 2-propanol. wt, wild type. (E) Transcription of adh2 and adh3 was detected by hybridization with ³²P-labeled adh2 and adh3 fragments, respectively, as probes. Total RNAs were prepared from wild-type cells grown on propane, 1-propanol, and 2-propanol.