TABLE 4.

Substrate specificities of purified Adh1, Adh2, and Adh3

Activity and substrate	Relative activitya (%)
	Adh1	Adh2	Adh3
Primary alcohol oxidation
Methanol	NDb	ND	ND
Ethanol	25.6	22.9	ND
1-Propanol	21.4	32.6	ND
1-Butanol	50.3	ND	ND
1-Pentanol	14.8	ND	ND
1-Hexanol	ND	ND	ND
1-Heptanol	ND	ND	ND
1-Octanol	ND	ND	ND
Secondary alcohol oxidation
2-Propanol	100	100	100
2-Butanol	119	82.8	62.9
(R)-(−)-2-Butanol	13.7	165	44.6
(S)-(+)-2-Butanol	178	125	78.7
2-Pentanol	129	40.0	63.5
2-Hexanol	19.2	12.7	78.1
2-Heptanol	ND	ND	109
2-Octanol	ND	ND	112
Ketone reduction
Acetone	36.9	54.9	34.9
2-Butanone	65.1	64.9	30.6

^a100% relative activity was defined as the activity with 2-propanol as the substrate.

^bND, activity of <5%.