FIGURE 4.

¹H-¹⁵N HSQC spectra of active peptide T3–778 (left) and control peptide T3–785 (right). Peptide sequences are shown at the top with ¹⁵N/¹³C-labeled residues underlined. The red circle highlights the chemical shifts of residue Ile/Leu at the collagenase cleavage site. For active peptide T3–778, the chemical shift of Ile in chain 1 (^1TI17) is different from Ile in chains 2 and 3. The peaks corresponding to the monomer and trimer state are denoted with a superscript M or T. The superscripted number 1, 2, and 3 corresponds to the leading, middle and lagging chains, respectively.