TABLE 1.
Triple helical peptides modeling the collagenase cleavage sites and their activities
Residues that are 15N labeled are underlined; each peptide has five labeled residues including a Gly at the central position in its (GPO)5/(GPO)3 C-terminal end; mutated residues relative to peptide T3–778 are bolded in peptides T3–778[I-L], T3–778[IT-TO], and T3–778[P-A].
| Peptide | Sequence | kcat/Km |
|---|---|---|
| m−1s−1 | ||
| T3–778 | (GPO)4-GPL-GIA-GIT-GAR-(GPO)5GY | 1253 ± 249 |
| T3–778[I-L] | (GPO)4-GPL-GLA-GIT-GAR-(GPO)5GY | 1562 ± 130 |
| T3–778[IT-PO] | (GPO)4-GPL-GIA-GPO-GAR-(GPO)5GY | 883 ± 134 |
| T3–778[P-A] | (GPO)4-GAL-GIA-GIT-GAR-(GPO)5GY | 125 ± 50 |
| T3–785 | PO(GPO)2-GIT-GAR-GLA-GPO-(GPO)3GY | 173 ± 81 |