TABLE 1.

Identification of tryptic DnaJ fragment by MS

Proteolysis was performed at 1:40 trypsin:DnaJ molar ratio for 5 min. Reaction was stopped by addition of 1 mm PMSF, and the fragment masses were obtained by LC-MS, as described under “Experimental Procedures.”

DnaJ fragment	Molecular mass
	Experimental	Theoretical
	kDa	kDa
109–376	29132.2	29134
111–376	28847.9	28850
114–376	28549.5	28551
154–359	22143.2	22144
174–376	22067.2	22068
190–376	20278.1	20279