FIGURE 5.

Cytoplasmic retention of RAR by CART1. NIH3T3 cells were transfected with HcRed-mRARβ and GFP-CART1 (or its variants) alone or together and treated with 1 μm AtRA. Cells were then fixed and observed under a fluorescence microscope. DAPI (1 μg/ml) was used to localize chromosomal DNA in the nucleus. A and B, localization of HcRed-RARβ alone (A) and together with GFP-CART1 (B) is shown. C and D, localization of CART1 C-terminal truncation (CART1 ΔC: amino acids 1–719) alone (C) and together with RAR (D) is shown. E and F, localization of the C-terminal region of CART1 (CART1C: amino acids 699–756) alone (E) and together with RAR (F) is shown.