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. 2010 Oct;8(5):553–570. doi: 10.1089/adt.2010.0321

Fig. 2.

Fig. 2.

High-content image acquisition and data analysis. Analysis of images acquired from INCA3000. HEK293 cells (4,000 cells per well) treated with compound and inoculated with DENV-2 (MOI of 0.5) were evaluated for antiviral activity at 48 h postinfection. (A) Distribution plot and image of DENV E fluorescence intensity in a well treated with vehicle-only 1% dimethyl sulfoxide (v/v). (B) Distribution plot and image of DENV E fluorescence intensity in a well treated with 10 μM of moderately effective inhibitor. (C) Distribution plot and image of DENV E fluorescence intensity in a well treated with 4 μg/mL of carrageenan. Plates were fixed and stained as described in the Materials and Methods section. Images were acquired using INCA3000, with green channel for DENV E and blue channel for detection of Hoechst-stained nuclei.