TABLE 1.
Strains and plasmids used
| Strain or plasmid | Genotype or description | Source, construction, or reference |
|---|---|---|
| Strains | ||
| 14028 | Wild-type S. enterica serovar Typhimurium | American Type Culture Collection |
| AT351 | 14028 flhD::Tn10 | 14028 × P22/KK2040 (40) |
| BA460 | 14028 hilA1550::MudJ flhD::Tn10 | BA1550 × P22/AT351 |
| BA461 | 14028 hilA1550::MudJ flhD::Tn10 sirA3::Cam | BA1750 × P22/AT351 |
| BA736 | 14028 sirA2::Kan | 2 |
| BA743 | SL1344 sipC11-6::Tn5 lacZYA sirA3::Cam | EE638 × P22/BA705 (2) |
| BA746 | 14028 sirA3::Cam | 2 |
| BA790 | SL1344 ΔhilA520 sirA3::Cam | 2 |
| BA1550 | 14028 hilA1550::MudJ | 2 |
| BA1750 | 14028 hilA1550::MudJ sirA3::Cam | 2 |
| BL21λDE3 | E. coli ompT hsdSB (rB− mB−) gal dcm λDE3 | Novagen |
| BW20767 | E. coli leu-63::IS10 recA1 creC510 hsdR17 endA1 zbf-5uidA(ΔMluI)::pir+thi RP4-2-tet::Mu-1kan::Tn7 | 50 |
| EE638 | SL1344 sipC11-6::Tn5 lacZYA | 7 |
| RG206 | 14028 flhD::Tn10 sirA2::Kan | AT351 × P22/BA736 |
| RG235 | 14028 csrB+/csrB::lacZYA integrant | BW20767/pRG84 mated with 14028, with selection on M9 minimal glucose plates with kanamycin |
| SL1344 | S. enterica serovar Typhimurium hisG rpsL xyl | 30 |
| TIM25 | 14028 csrA+/csrA::lacZYA integrant | BW20767/pMT6 mated with 14028, with selection on M9 minimal glucose plates with kanamycin |
| TIM26 | 14028 sirA3::cam csrA+/csrA::lacZYA integrant | TIM25 × P22/BA746 |
| TIM27 | 14028 sirA3::cam csrB+/csrB::lacZYA integrant | RG235 × P22/BA746 |
| TOP10 | E. coli mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 deoR recA1 araD139 Δ(ara-leu)7697 galU galK rpsL endA1 nupG | Invitrogen |
| XL1-Blue | E. coli recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacIqZΔM15 Tn10] | Stratagene |
| Plasmids | ||
| pBA322 | pBAD18-kn sirA+ | This study (see Materials and Methods) |
| pBA416 | pET24a sirA expression vector | This study (see Materials and Methods) |
| pBAD18-kn | Arabinose-conditional expression vector; Kanr ColE1 | 26 |
| pBAD33 | Arabinose-conditional expression vector; Camr p15A | 26 |
| pCR2.1-TOPO | Cloning vector; Kanr ColE1 | Invitrogen |
| pCR-Blunt II-TOPO | Cloning vector; Kanr ColE1 | Invitrogen |
| pCR-Script AMP | Cloning vector; Ampr ColE1 | Stratagene |
| pET24a | Expression vector; Kanr ColE1 | Novagen |
| pMT6 | csrA-lacZYA fusion in pVIK112 | This study (see Materials and Methods) |
| pQE30 | Expression vector; Ampr ColE1 | Qiagen |
| pRG19 | motA::luxCDABE fusion in pSB401 | 24 |
| pRG38 | flhD::luxCDABE fusion in pSB401 | 24 |
| pRG61 | sopB::luxCDABE fusion in pSB377 | This study (see Materials and Methods) |
| pRG67 | pQE30 barA198 expression vector | This study (see Materials and Methods) |
| pRG84 | csrB-lacZYA fusion in pVIK112 | This study (see Materials and Methods) |
| pSB377 | luxCDABE transcriptional fusion vector; Ampr ColE1 | 67 |
| pSB401 | luxCDABE transcriptional fusion vector; Tetr p15a | 67 |
| pJVR4 | pBAD33 sirA+ | 2 |
| pVIK112 | lacZYA transcriptional fusion vector; Kanr oriR6K | 35 |