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Growth profile of CHO-233 cells expressing p27Kip1 or sT. Cell lines CHO-23393 and CHO-233176 harboring the dual regulation plasmid pMF233 were seeded in triplicate cultures at 105 cells/ml under three different conditions: p27Kip1OFF/sTOFF (+Tet+PI), p27Kip1OFF/sTON (+Tet/–PI) and p27Kip1ON/sTOFF (–Tet/+PI), where +Tet and +PI are cultures containing tetracycline and pristinamycin, respectively (Fig. 1).
