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Growth profiles of CHO-226 cells expressing p27Kip1S or antisense p27Kip1AS. Three clones (CHO-22696, CHO-226100, CHO-226108) harboring the plasmid pMF226 (PhCMV*-1-p27Kip1-PPIR) were seeded in triplicate cultures at an initial population of 105 cells/ml under three different conditions: p27Kip1SOFF/p27Kip1ASON (+Tet/–PI), p27Kip1SON/p27Kip1ASOFF (–Tet/+PI), or p27Kip1SOFF/p27Kip1ASOFF (+Tet/+PI, complete repression of both genes).
