Figure 2. Different methods may enrich for different origins.

A | Mapping the positions of small nascent leading strands (SNSs). Genomic DNA from proliferating cells is denatured and single-stranded DNA (ssDNA) is size-fractionated, usually by sucrose density gradient centrifugation. Strands that are larger than Okazaki fragments (50–350 bp) but small enough to be representative of initiation sites (usually 500–1,500 bp) are isolated. Genomic DNA is isolated from cells synchronized in G1 phase by sorting cells with an unreplicated DNA content using a fluorescence-activated cell sorter (FACS). G1 DNA is used as a reference for microarray analysis because all sequences have an identical copy number. SNSs and genomic DNA are then differentially labelled and hybridized to a microarray or directly subjected to deep sequencing. In the microarray schematic, the colours represent the efficiencies of the origins. In principle, the degree of enrichment of probe sequences in the SNS preparation should be proportional to the fraction of cells in which initiation takes place close to any given probe, but contamination from broken, unreplicated DNA must be carefully controlled. B | Principle of the bubble-trap method⁶¹. Ba | Initiation of DNA creates replication bubble structures that essentially behave as circular molecules. Hence, when genomic DNA is digested with a restriction enzyme and then mixed with molten agarose and allowed to cool to form an agarose plug, recently initiated origin-containing fragments become trapped in the polymerized agarose. Bb | The plug is then subjected to exhaustive electrophoresis, which removes all unreplicated fragments as well as replicating fragments containing branched structures resulting from the entry of replication forks initiated outside the DNA fragment. Bc | Origin-containing DNA within the plug is then isolated and cloned. Bd | This cloned DNA can be either hybridized to a microarray or sequenced. C | Types of origins enriched by each method. Vertical lines depict restriction enzyme cutting sites and red circles depict the positions of replication origins, simplified as two types termed efficient or inefficient. The following describes which method would most easily detect the activity of origins positioned as shown. Note that replicate experiments using different restriction enzymes can overcome some of the limitations of the bubble-trap method. Ca | SNSs but not bubble trap. The origin is efficient and localized but the fragment is small and any bubbles formed will be quickly converted to branched or linear structures. Cb | Bubble trap but not SNSs. The individual origins fire too infrequently for their localized sites to be detected by SNSs, but most of them will make bubble structures that will trap the fragment regardless of where initiation occurs. Cc | Bubble trap and SNSs. The right-most origin will be detected by SNSs and the collection of origins will make detectable bubbles. Cd | Bubble trap and SNSs. Because it is an efficient, localized origin, it will be detected by SNS, and because it is positioned at the centre of a sufficiently large restriction fragment, it will generate bubble structures. Ce | Bubble trap, but may be difficult for either method. The origins are too inefficient for SNS detection. For bubble trap, large restriction fragments can be retained false-positives. Large fragments are also more difficult to clone into a plasmid library. Cf | SNSs but not bubble trap. These efficient origins can be detected by SNSs but are too close to the edge of the fragment to be detected by the bubble-trap method. When initiation occurs off-centre, the fragment rapidly converts to a branched structure when the replication fork crosses the restriction site. Cg | Neither method. The origin is too inefficient for detection by SNSs and the fragment is too small for detection by bubble traps. Part B is modified, with permission, from REF. 61 © (2009) Springer.