Figure 3. Drop in egg potential and decrease in specific polyadenylated transcripts upon egg aging in batches of eggs obtained from different frogs.

Eggs were fertilized immediately or with a 3-hour delay. (A) The developmental competence refers to the survival rate (white boxes) and the percentage of defective development (grey boxes) observed at the free swimming larval stage 40 (20). The defective development includes malformation (dark grey) as well as undersized and retarded development (light grey) as exemplified in Fig. 1B and C. Experiment (exp.) 6 and the experiment in Fig. 2 were made ten months apart using the same female. (B) From each experiment the aging induced decrease determined by oligo(dT) qRT-PCR of the fourteen polyadenylated transcripts is given in the same order as analyzed in Fig. 2.