Table 1. Processing site and protease mutations incorporated into pGPfs-1C.

Processing site	Nucleotide sequence	Amino acid sequence
p17/p24 WT	CAA AAT TAC/CCT ATA GTG	Gln Asn Tyr/Pro Ile Val
p17/p24 Mut	CAA AAT ATC/CTT ATA GTG	Gln Asn Ile/Leu Ile Val
p24/p2 WT	AGA GTT TTG/GCT GAA GCA	Arg Val Leu/Ala Glu Ala
p24/p2 Mut	AGA GTT GTG/GCT GAA GCA	Arg Val Val/Ala Glu Ala
NC/TF WT	CAG GCT AAC/TTC CTC CGC	Gln Ala Asn/Phe Leu Arg
NC/TF Mut	CAG GCT ATC/GAT CTC CGC	Gln Ala Ile/Asp Leu Arg
TF440/441 WT	TTG GCC TTC/CTA CAA GGG	Leu Ala Phe/Leu Gln Gly
TF440/441 Mut	TTG GCC ATC/CTA CAA GGG	Leu Ala Ile/Leu Gln Gly
TF/Protease WT	TTT AAC TTC/CCT CAG GTC	Phe Asn Phe/Pro Gln Val
TF/Protease Mut	GCG GCC GCC/CCT CAG GTC	Ala Ala Ala/Pro Gln Val
Protease/Pol WT	TTA AAT TTT/CCC ATT AGC	Leu Asn Phe/Pro Ile Ser
Protease/Pol Mut	TTA AAT TTT/GCTGCAGCC	Leu Asn Phe/Ala Ala Ala
Pol/RNaseH WT	GAA ACC TTC/TAT GTA GAT	Glu Thr Phe/Tyr Val Asp
Pol/RNaseH Mut	GAA ACC ATC/TAC GTA GAT	Glu Thr Ile/Tyr Val Asp
RNaseH/Integrase WT	AAA GTA CTA/TTT TTA GAT	Lys Val Leu/Phe Leu Asp
RNaseH/Integrase Mut	AAA GTA ATA/TTT TTA GAT	Lys Val Ile/Phe Leu Asp