TABLE 3.

Comparisons of lasB, aprA and lasR transcript levels in early log phase (120 min) and stationary phase (300 min) for P. aeruginosa strains

Strain no. and origina	Amt of transcript at indicated culture timeb						Comparison with lasR gene of PAO1
	lasR transcript		lasB transcript		aprA transcript
	120 min	300 min	120 min	300 min	120 min	300 min
47, B	0.24	1.94	0.04	3.46	1.7	197.93	Full identity
63, E	0.24	4.09	0.6	1.74	0.09	3.23	C201 stopc
41, B	0.45	0.66	0.04	0.01	1.03	6.93	Full identity
45, B	0.59	4.14	1.51	13.41	0.17	0.57	Silent mutations
53, B	0.88	10.36	1.43	1.44	4.5	12.91	Insert
42, B	0.92	1.67	2.58	4.38	0.13	1	Q186P
58, E	1.09	2.59	0.27	18.94	0.1	11.78	Silent mutations
55, B	1.36	17.66	0.83	0.46	1.83	3.11	Full identity
43, B	1.64	7.77	0.16	0.17	0.23	1.81	A70E
37, B	2.26	8.09	1.59	20.92	2.85	64.95	Silent mutations
30, P	2.42	9.11	0.46	12.14	0.29	29.62	Silent mutations
67, E	2.76	3.21	1.27	0.58	1.08	3.64	Full identity
15, P	2.96	5.58	0.6	35.98	1.42	309.28	Silent mutations
51, B	3.19	7.38	2.47	3.35	15.59	17.21	Full identity
66, E	3.62	6.49	3.02	2.63	5.09	9.56	Insertions
24, P	3.93	44.01	0.87	27.61	0.88	111.67	Silent mutations
48, B	4.02	5.16	15.05	9.86	2.14	1.72	F210L
16, P	4.83	12.93	0.25	4.74	0.86	44.89	Full identity
39, B	5.15	27.22	0.96	7.73	0.88	21.5	Silent mutations
11, P	6.86	27.99	0.49	5.07	0.29	33.74	Multiple substitutionsd
64, E	7.45	28.91	1.87	201.68	4.51	144.01	Silent mutations
23, P	9.54	88.21	3.01	87.95	4.25	141.91	Silent mutations
71, E	11.35	12.94	0.5	6.36	0.34	35.45	Multiple substitutions
20, P	11.55	6.2	2.59	0.61	6.95	2.01	Full identity
50, B	12.67	26.98	2.27	42.86	0.41	134.41	K25M
65, E	14.7	70.88	1.7	121.08	3.38	361.46	Full identity
69, E	15.24	19.47	7.51	119.05	7.69	197.64	Silent mutations
36, P	15.84	33.05	3.06	0.44	0.45	4.78	Insertions
19, P	18.57	44.88	10.01	8.84	3.31	31.67	A50G
38, B	21.24	35	1.73	155.55	2.12	203.25	Silent mutations
9, P	22.15	51.88	2.32	73.68	1.22	3.24	Full identity
14, P	34.27	37.36	1.53	3.14	0.94	9.12	Full identity
13, P	37.21	25.97	2.49	77.86	0.3	59.51	V226I
61, E	42.1	137.97	4.57	58.77	6.48	133.12	Full identity
27, P	106.29	414.39	5.02	258.05	16.84	269.79	Silent mutations
PAO1	8.39	12.23	8.22	129.07	5.12	210.15	Full identity

^aB, strain isolated from patient with bacteremia; P, strain isolated from patient with pneumonia; E, strain isolated from the environment.

^blasB, aprA, and lasR transcript levels were normalized by using the ratio of the amount of these transcripts to the small-subunit rRNA level and multiplying by 1,000.

^cAmino acid substitution.

^dStrains 11 and 71 had the following amino acid substitutions: R66K, N136S, A137N, and G172N.