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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Neuroimage. 2010 Aug 20;54(1):113–122. doi: 10.1016/j.neuroimage.2010.08.033

Table 1.

Densitometry of plaques and tissue in APP/PS1 and WT brain sections stained for iron.

APP/PS1
 Plaq./Tiss. APP/PS1 Plaq. WT
APP/PS1 Tiss. vs.
Region Plaquea Tissuea Ratio vs Tiss p-valueb Tissuea
WT Tiss. p-value c
Cortex 153.6 ± 2.6
ns		 222.8 ± 1.8 0.69 ± 0.01 0.001 219.2 ± 0.4
Hippocampus 153.8 ± 2.8
ns		 218.7 ± 1.0 0.70 ± 0.01 0.001 215.9 ± 0.8
Thalamus 11.8 ± 0.2
0.001		 215.7 ± 2.1 0.055 ± 0.001 0.001 201.4 ± 1.4
a

Mean (± SEM) gray level value of n=10 plaques or adjacent tissue ROIs per region measured in 8-bit gray scale images of DAB-enhanced Prussian blue stained histological sections from a 24-month old APP/PS1 or age-matched WT mouse. Lower gray level value indicates darker staining and relatively higher iron content.

b

Two-way ANOVA APP/PS1 Plaque vs. Tissue Iron Densitometry: Plaque vs. Tissue – F(1,59)=4874.35, p<0.001; Region - F(2,59)=921.83, p<0.001; Plaq./Tiss. × Reg. Interaction – F(2,59)=799.64, p<0.001; Student-Newman-Keuls multiple comparisons.

c

Two-way ANOVA APP/PS1 Tissue vs. WT Tissue Iron Densitometry: AD vs. WT – F(1,59)=37.13, p<0.001; Region - F(2,59)=42.50, p<0.001; AD/WT × Reg. Interaction – F(2,59)=10.72, p<0.001; Student-Newman-Keuls multiple comparisons.