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. 2001 Feb 15;29(4):e20. doi: 10.1093/nar/29.4.e20

Figure 3.

Figure 3

Amplification of tester-specific TSC-22R (A) and TSPY intron sequences (B). Genomic DNA of CTL (lane 1) and My-La (lane 2), cDNA of CTL (lane 3) and My-La (lane 4) and non-template DNA (lane 5) were amplified with a pair of primers that was either derived from a TspRI fragment of TSC-22R cDNA (A) or a TSPY intron (B) sequence and electrophoretically separated on an ethidium bromide-stained 1.5% agarose gel. M, 100 bp ladder size marker. The calculated size of genomic and cDNA TSC-22R amplification products was 1520 and 370 bp, respectively. The calculated size of the TSPY intron fragment was 220 bp.