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. 2001 Feb 15;29(4):e20. doi: 10.1093/nar/29.4.e20

Figure 4.

Figure 4

Amplification of HLA class II and flanking region genes. Genomic DNA of CTL (lane 1) and My-La (lane 2), cDNA of CTL (lane 3) and My-La (lane 4) and non-template DNA (lane 5) were amplified with pairs of primers that were derived from the indicated genes Tap2 (calculated sizes: 2197 and 345 bp for genomic DNA and cDNA, respectively), HLA-DQα (763 and 351 bp for genomic DNA and cDNA, respectively), HLA-DRB1 (1728 bp for genomic DNA), HLA-DRα (165 bp for cDNA) and Notch4 (329 and 209 bp for genomic DNA and cDNA, respectively). Amplification of genomic DNA and cDNA with primers from HLA-DRα (calculated size: 904 bp) and HLA-DRB1 (250 bp), respectively, were not shown but confirmed the shown results. The PCR products were electrophoretically separated on a 1.5% agarose gel and stained with ethidium bromide. M, 100 bp ladder size marker.