Table 3.
Association equilibrium constants measured for tolbutamide at Sudlow sites I and II at pH 7.4 and 37°Ca
| Tolbutamide | Sudlow Site I (R-warfarin) (× 104 M−1) | Sudlow Site II (L-tryptophan) (× 104 M−1) |
|---|---|---|
| Normal HSAb | 5.5 (± 0.2) | 5.3 (± 0.2) |
| gHSA1 | 6.9 (± 0.2) | 5.9 (± 0.3) |
| gHSA2 | 6.6 (± 0.5) | 7.2 (± 0.3) |
| gHSA3 | 6.5 (± 0.2) | 6.4 (± 0.3) |
a
The values in parenthesis represent a range of ± 1 S.D for the population of results. These values were determined by using error propagation and the standard deviations of the slopes and intercepts that were obtained from best-fit lines generated according to Eqn. (6).
b
The results provided for normal HSA are based on previous work described in Ref. [30] and were acquired using experimental conditions identical to those used in this current report for glycated HSA.