Skip to main content
. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Exp Eye Res. 2010 Aug 17;91(5):652–659. doi: 10.1016/j.exer.2010.08.011

Figure 1.

Figure 1

Cellular tropism of AAVrh8 and AAVrh10 vectors in the mouse retina. (A) AAVrh8 transduced cells in the GCL and cells in the lower third of the INL (white arrowhead), which correspond to amacrine cells by location and morphology. While rare, some cells resembled bipolar cells by location and morphology (black arrowhead). There were apparent horizontal cell somata and processes in the OPL transduced with EGFP (white arrow). There were also processes from Müller cells extending up to the OLM (black arrow). Punctate EGFP expression, consistent with synapses, was also seen throughout the IPL. The inset (right) shows a higher magnification image with increased contrast to more clearly see the punctate expression in the IPL. Scale bars = 20 μm. (B) A lower magnification image of a retinal cross section transduced by AAVrh8 shows extensive regional labeling in the INL (double asterisks). Scale bar = 40 μm. (C) Consistent with ganglion cell transduction, there were EGFP-positive ganglion cell axons (black arrowheads). Müller cell processes can be seen projecting up to the OLM (black arrow) as well as some photoreceptor somata (white arrowhead). Scale bar = 40 μm. (D) Example of a control retina (sham injected with saline) with no EGFP. Scale bar = 20 μm. (E) EGFP levels were higher in retinas injected with AAVrh10. Transduction was seen in the GCL and throughout the INL. The OPL and apparent horizontal cells were strongly transduced with high levels of EGFP expression. There were also some labeled photoreceptor cell bodies (white arrowhead and insets) and Müller cells extending toward the OLM (black arrow). The inset directly above the white arrow shows a higher contrast image of the photoreceptor indicated in the image. In inset right shows a high contrast image of a region of several transduced photoreceptors from a different retinal region. Some cells in the RPE also appeared transduced and EGFP-positive axons were seen in the GCL suggesting ganglion cell transduction (black arrowhead). Scale bar = 20 μm. (F) Typical transduction pattern for AAVrh10 showing extensive EGFP expression in the OPL (double asterisks) and more patchy expression in the inner retina. The overall AAVrh8 transduction pattern was similar to AAVrh10, but with less intense EGFP expression in the OPL and more regional expression in the inner retina (Figure 1B). Scale bar = 80 μm.