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Quantification of in vitro transgene expression from transcriptional amplification-enhanced Tet-Off regulatory systems at MOIs of 1, 5, 15 and 25. (A) SYN-containing system in neuronal PC12 cells. (B) GfaABC1D-containing system in astroglial 1321N1 cells. Numbers of DsRed2-positive cells per field of view were counted under 100× magnification. Six fields were selected randomly for cell counting. The error bars indicate the standard deviations
