Figure 7.
L162313 (AT1 receptor agonist) reduces GLT-1 protein expression at a transcriptional level. (A) L162313 treatment decreased GLT-1 expression induced by 4 days of ADM culture. Simultaneous 4 day treatment with the proteasome inhibitor lactacystin (0.3 µM) did not attenuate L162313 (3 µM)-induced GLT-1 down-regulation in ADM-cultured astrocytes. (B) GLT-1 mRNA level was increased by ADM culture (4 days) and was decreased by L162313 (0.1–3 µM) to 8.5 ± 0.7 in a concentration-dependent manner. Data represent mean ± SE, n= 3 for each group. ###P < 0.001 versus control; **P < 0.01, ***P < 0.001 versus ADM; anova followed by Newman–Keuls multiple comparison test. n.s., not significant; lacta, lactacystin; L162, L162313.