Table 1.
Analysis of oxidation products from l-amino acids. Twenty micromoles of each l-amino acid was incubated with 15.5 × 10−3 U of l-AAO under standard conditions of l-amino acid oxidation, and the reaction mixture was applied to HPLC with a TSKgel DEAE-5PW column. Molecular mass of reaction products was analyzed by an HCT Ultra mass spectrometric instrument.
| l-Amino acid | Elution time (min) | Reaction product | |
|---|---|---|---|
| l-Amino acid | Products | Molecular mass (product name) | |
| l-Homoserine | 3.3 | 15.8 | 118.1 (2-oxo-4-hydroxybutyric acid) |
| l-Leucine | 3.2 | 18.3 | 130.0 (2-oxo-4-methylvaleric acid) |
| l-Lysine | 2.7 | 4.8 | 145.1 (2-oxo-6-aminohexanoic acid) |
| l-Glutamine | 3.3 | 15.1 | 145.1 (2-oxo-4-carbamoylbutanoic acid) |
| l-Arginine | 2.3 | 3.3 | 173.1 (2-oxo-5-guanidinopentanoic acid) |
| l-Glutamic acid-Na | 14.6 | 18.5 | 190.0 (2-oxoglutaric acid) |
| l-Citrulline | 3.2 | 14.9 | 174.0 (2-oxo-5-ureidovaleric acid) |
| 15.8 | 174.0 (pyrrolidine-1-carbamyl-2-hydroxy-2-carboxylic acid) | ||
| l-Phenylaranine | 3.5 | 24.9 | 164.1 (2-oxo-3-phenylpropionic acid) |
| l-Histidine | 3.0 | 17.9 | 154.3 (2-oxo-4-imidazolepropionic acid) |
| Nε-Acetyl-l-lysine | 3.2 | 15.5 | 187.1 (2-oxo-6-acetylaminohexanoic acid) |