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. Author manuscript; available in PMC: 2010 Oct 24.
Published in final edited form as: Chembiochem. 2008 Sep 22;9(14):2200–2203. doi: 10.1002/cbic.200800208

Figure 2.

Figure 2

Relative peptide affinities. A) Radiolabeled Gαi1 was incubated with immobilized, biotinylated R6A and varying concentrations of either R6A (▼), unmodified peptide (▲), or N-Me-K (◆) for 3 h. at 4°C. The radiolabel bound was normalized to that which bound in the absence of competitor. The plotted values represent the mean of three experimental values and the error bars correspond to the standard deviation. B) Experimentally determined binding data is shown above. The error is calculated based on the standard error of the mean (SEM) for the log IC50 using GraphPad 5.0.