Table 3.
Effect of in vitro stimulation with collagen I (CI) on gene expression in cultured peripheral blood mononuclear cells (PBMC) based on comparison of matched samples (PBMC from the same volunteers, cultured with CI versus cultured without CI) in healthy controls (Ctrls), patients with early dcSSc (Early) and patients with late dcSSc (Late). Significant fold differences (P < 0·05) are shown. Positive values indicate fold higher and negative indicate fold lower levels of gene expression in indicated CI-stimulated cultures compared with cells cultured in control medium (M).
| Gene | UniGene | OMIM | Full gene name | CI versus M Ctrls | CI versus M Early | CI versus M Late |
|---|---|---|---|---|---|---|
| IFN-β | Hs.93177 | 147640 | Interferon β1 | +1·6 | ||
| IL11 | Hs.467304 | 147681 | Interleukin 11 | −1·7 | ||
| SAA1 | Hs.632144 | 104750 | Serum amyloid A1 | +2·6 | ||
| TLR7 | Hs.659215 | 300365 | Toll-like receptor 7 | +1·5 | ||
| TLR8 | Hs.660543 | 300366 | Toll-like receptor 8 | +1·6 | ||
| TRAF1 | Hs.531251 | 601711 | TNF receptor-associated factor 1 | +1·6 | ||
| RUNX2 | Hs.535845 | 600211 | Runt-related transcription factor 2 | +2·9 | +2·1 | |
| EMAP-2 | Hs.591680 | 603605 | Endothelial monocyte-activating polypeptide 2 | +3·1 | +5·8 | |
| EGR2 | Hs.1395 | 129010 | Early growth response 2 | +1·8 | ||
| FOSL1 | Hs.283565 | 136515 | FOS-like antigen 1 | +1·6 | ||
| JAK2 | Hs.656213 | 147796 | Janus kinase 2 | +2·2 |