Fig. 7.

The inhibitory effect of immune receptor expressed on myeloid cells 1 (IREM-1) on B cell activation factor (BAFF)-mediated signalling is effective in U937 cells. U937 cells were pretreated for 30 min with 1 µg/ml of mouse IgG (M)/anti-IREM-1 mAb (IR) or 5 µM of TAT (T)/TAT–YADL (T-Y). The cells were then stimulated with 1 µg/ml of anti-BAFF monoclonal antibody. Culture supernatants were collected 24 h after activation and the concentration of interleukin-8 was measured using enzyme-linked immunosorbent assay and matrix metalloproteinase (MMP)-9 activity was measure using gelatin zymogram. These experiments were repeated twice, with essentially the same results.