Fig. 5.

Cathelicidin anti-mycobacterial activity of cathelin-related anti-microbial peptide (CRAMP) and LL-37. (a) The immunolectronmicroscopy study shows strong CRAMP immunolabelling and dissolution of the cell wall (arrows) of mycobacteria incubated in vitro with this anti-microbial peptide (magnification ×75 000). (b) Various concentrations of each peptide were incubated with Mycobacterium tuberculosis strain H37Rv (1 × 10³M. tuberculosis at 37°C in 100 µl 7H9 broth), colonies were counted after 21 days of incubation at 37°C to determine minimum inhibitory concentrations. Bars represent mean values ± standard deviation.