Fig. 4.

Representative dot plots showing interferon (IFN)-γ, interleukin (IL)-2 and tumour necrosis factor (TNF)-α production by CD8⁺ and CD8^– (CD4⁺) T cells from blood from a stable patient and a lung transplant patient with declining forced expiratory volume in 1 s (FEV₁) (D). T cells were identified by CD3 phycoerythrincyanin 5 (PC5)versus side-scatter characteristics. There was a significant increase in blood CD8⁺ and CD8^– (CD4⁺) T cell IFN-γ, IL-2 and TNF-α in patients with declining FEV₁ compared with the stable patient group.