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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2010 Jun 10;30(9):1758–1765. doi: 10.1161/ATVBAHA.110.206045

Figure 1.

Figure 1

Decreased iNKT cell numbers and functions in apoE−/− mice. A, iNKT cell percentages in the thymus, spleen, and liver of B6 and apoE−/− mice. Shown are representative dot plots of 3 experiments with 3 mice per group. B, Absolute iNKT cell numbers in the spleen and thymus. Bars represent the mean and SE. Data are representative of 3 experiments. C, Flow cytometry analyses of NK1.1 and CD44 on iNKT cells from the thymus of B6 and apoE−/− mice. D, Splenocytes from B6 and apoE−/− mice were cultured for 72 hours in the presence of the indicated concentrations of α-GalCer. IFN-γ and IL-4 were determined by ELISA. Data points represent the mean and SE of 3 mice in each group. Shown is 1 representative experiment of 3. E, Mice were injected IP with 4 µg α-GalCer, and cytokine production was determined by ELISA. Serum IFN-γ was measured 24 hours after injection, and IL-4 was measured 2 hour after injection. Shown are the mean and SD of 3 mice per group. F, Mice were injected with 4 µg α-GalCer, and 24 hours after injection, activation of splenic B cells, NK cells, T cells, and DCs was determined by flow cytometry. Shown are representative histograms of 5 mice in each group. *P<0.05 as determined by Student’s t test.