Figure 2.
Decreased cytokine production is iNKT cell specific. Splenocytes from B6 and apoE−/− mice were stimulated for 4 hours in vitro with 25 ng/mL of α-GalCer, and intracellular IFN-γ and IL-4 were determined by flow cytometry. A, Representative dot plots from 3 experiments. B, Percentages of IFNγ+ (left panel) and IL-4+ (right panel) iNKT cells. *P<0.05 as determined by Student’s t test.