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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2010 Jun 10;30(9):1758–1765. doi: 10.1161/ATVBAHA.110.206045

Figure 3.

Figure 3

iNKT cells from apoE−/− mice display an anergic phenotype. A, B6 and apoE−/− mice were injected with 4 µg α-GalCer, and splenic iNKT cells were analyzed by flow cytometry at 0 hours, 24 hours, 3 days, 7 days, and 1 month following injection. Shown are representative dot plots of 3 mice per group gating on B220− cells. B, Flow cytometry data as shown by percent tetramer+ cells (gated on B220−TCRβint; top panel). Fold change over baseline in percent tetramer+ cells (bottom panel). C, B6 and apoE−/− mice were injected IV with 350 µg ConA in 200 µL PBS and killed 24 hours later. ConA-induced hepatitis (black arrowheads show areas of lymphocyte infiltration) was assessed by hematoxylin and eosin staining of paraffin-embedded liver sections. Shown are representative sections from 4 mice per group.