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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: Cancer Cell. 2010 Sep 14;18(3):268–281. doi: 10.1016/j.ccr.2010.08.006

Figure 8. The NEMO/IKK complex activity is essential for the maintenance of T-ALL in vivo.

Figure 8

(A–B) Peripheral blood FACS analysis using CD4, CD8 and Ly5.2 antibodies at different time points post transplantation. (C) ΔEN1-luc Nemowt/wt, Mx-Cre and ΔEN1-luc Nemof/f, Mx-Cre chimeras were monitored before and after pI-pC injections using in vivo imaging system (IVIS) to quantify luciferase intensity. 2 weeks post-transplantation and before any deletion of NEMO, both chimeras harbored homogenous tumor load. Once this was verified mice were injected by pI-pC. (D) Quantification of luciferase intensity (dorsal and ventral) at different time-points. Error bars denote +/− Standard Deviation. (E) Determination of apoptotic cells gated on GFP+ cells 5 days after pIpC injections in ΔEN1-GFP Nemowt/wt, Mx-Cre and ΔEN1-GFP Nemof/f, Mx-Cre chimeras. (F) Micrographs of hematoxylin/eosin-stained liver and spleen sections prepared 40 days after transplantation. (G) Kaplan-Meyer survival curve of the different recipient animals (p<0.005). See also related figure S8.