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. 2010 Jan;1(1):26–39. doi: 10.1177/1947601909358184

Figure 6.

Figure 6.

KIF18A regulates the stability of BubR1 and CENP-E during mitosis. (A) HeLa cells transfected with KIF18A or luciferase siRNA for 24 h were fixed and stained with antibodies to α-BubR1 (green) and CREST (red). DNA was stained with 4′6′-diamidino-2-phenylindole (DAPI; blue). Representative images are shown. Arrows indicate BubR1 signals on unaligned chromosomes. (B) HeLa cells transfected with KIF18A or luciferase siRNA for 24 h were fixed and stained with antibodies to CENP-E (green) and CREST (red). DNA was stained with DAPI (blue). Representative images are shown. (C) HeLa cells transfected with KIF18A, SGO1, or luciferase siRNA for 24 h were collected and lysed. HeLa cells treated with VP16 overnight were also used for lysate preparation. Equal amounts of proteins from various treatments were blotted for KIF18A, CENP-E, BubR1, Aurora B, Eg5, cyclin B, Mad2, PARP-1, and β-actin. R and A denote rounded-up cells and adherent cells, respectively. Arrow p-BubR1 denotes the phosphorylated BubR1. R and A denote rounded-up and adherent cells, respectively.