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. Author manuscript; available in PMC: 2010 Oct 25.
Published in final edited form as: Eur J Immunol. 2008 Feb;38(2):448–458. doi: 10.1002/eji.200737485

Figure 4. Changes in CD4+ T lymphocytes in the peripheral lymphoid organs post-L5Tx or sham surgery in BALB/c mice.

Figure 4

Total cells isolated from spleen and lumbar LNs of sham and L5Tx operated mice were labeled with mAbs and analyzed via FACS. Changes in the numbers of total splenocytes (A), numbers of splenic CD4+ T lymphocytes (CD3+CD4+) (B), and percentage of CD4+ T lymphocytes in the lumbar LNs (C) after surgeries are presented in the left column. Activation of splenic and LNs CD3+CD4+ cells were further determined through their surface expression of CD69, CD154 and CD45RB. Representative data are shown in D–F in the right column. Numbers of animals per group are indicated in each graph. Data are presented as mean ± SEM and “s” or “t” denote significant differences between selected time and day 0 within sham and L5Tx groups, respectively (p < 0.05).