. Author manuscript; available in PMC: 2010 Oct 25.

Published in final edited form as: Eur J Immunol. 2008 Feb;38(2):448–458. doi: 10.1002/eji.200737485

Table 1.

Combinations of primary Abs used for FACS

Experiment	Section in text	Primary Ab combination
i) Initial determination of lumbar spinal cord leukocyte infiltration	section 2.2	PE-anti-mouse CD45
ii) Phenotypic analysis of lumbar spinal cord infiltrating and splenic leukocytes	section 2.2 and section 2.3 data not shown	Tube 1) APC-Cy7-anti-mouse CD45, perCP (or PE-Cy7)-anti-mouse CD3, FITC-anti-mouse CD4, PE-anti-mouse CD8; Tube 2) APC-Cy7-anti-mouse CD45, perCP-anti-mouse CD3, FITC-anti-mouse panNK, PE-anti-mouse CD19; Tube 3) APC-Cy7-anti-mouse CD45, perCP-anti-mouse CD3, FITC-anti-mouse CD11b, PE-anti-mouse I-A^d.
iii) Phenotypic analysis of CD4⁺ T lymphocytes in spleen and lumbar lymph nodes	Section 2.3	Tube 1)APC-Cy7-anti-mouse CD45, PE-Cy7-anti-mouse CD3, FITC-anti-mouse CD4, Biotin-anti-mouse CD45RB; Tube 2)^a APC-Cy7-anti-mouse CD45, PE-Cy7-anti-mouse CD3, FITC-anti-mouse CD4, PE-anti-mouse CD40L, Biotin-anti-mouse CD69.

for lymph nodes, only tube 1 was used due to the limited numbers of cells available.

All mAbs were from BD Biosciences (San Diego, CA) except that Biotin-anti-mouse CD69 was from eBioscience (San Diego, CA).