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L. major WT-[pXG-GCVP-GFP] promastigotes expressing GCVP-GFP from the pXG-GCVP-GFP plasmid counter-stained with Hoechst 33342. Left panel, GFP fluorescence. Center panel, Hoechst fluorescence. Right panel, GFP and Hoechst fluorescence combined. All fluorescence images are shown overlaid on the phase-contrast image of the parasites.
